Gene finding: Prokaryotes vs. Eukaryotes
Eukaryotes
- Complex gene structures (exon/introns)
- D. melanogaster has an average of 4 introns/gene
- Very long genes (D. melanogaster X gene 160 kb)
- Very long introns
- Many introns
- �Nested�, overlapping, and alternatively spliced genes
- 5� UTRs with non-coding exons
- Long 3� UTRs
- Complex transcription machinery
- ORF-finding alone is not adequate