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Gene Information
cDNA	Gene	Function
GH17337	CG3400 (Annotation Report) FBgn0027621 (Gene Report) Map: 18C8-18C8	6-phosphofructo-2-kinase
Array Data (CG3400) Annotation MiniView (CG3400)

in situ Images and Annotations (GH17337)
Stage	Image (click thumbnail for full size image)	Body Part	Note	Comment
stage1-3		maternal		heavy staining but seems to be abundance
stage4-6		trunk mesoderm anlage in statu nascendi mesoderm anlage in statu nascendi mesectoderm anlage in statu nascendi visual anlage in statu nascendi ectoderm anlage in statu nascendi ventral ectoderm anlage in statu nascendi head mesoderm anlage in statu nascendi dorsal ectoderm anlage in statu nascendi anlage in statu nascendi subset procephalic ectoderm anlage in statu nascendi cellular blastoderm		punctate intense staining at cellular blastoderm- is it nuclear?
stage7-8		ventral nerve cord anlage procephalic ectoderm anlage mesectoderm primordium head mesoderm primordium P4 visual anlage posterior endoderm primordium P2 trunk mesoderm primordium P2 ventral ectoderm primordium P2 anterior endoderm anlage dorsal ectoderm primordium		some stripes. hard to capture becasue of intensity.. Seems to be almost ubiquitous expression until stage 10; then label disappears, stays longer in mesoderm and endoderm.
stage9-10		posterior endoderm primordium ventral ectoderm primordium anterior endoderm primordium ventral nerve cord primordium P3 mesectoderm primordium trunk mesoderm primordium dorsal ectoderm primordium crystal cell specific anlage head mesoderm primordium P2		stripes persist. Seems to be almost ubiquitous expression until stage 10; then label disappears, stays longer in mesoderm and endoderm.
stage11-12		posterior midgut primordium anterior midgut primordium procrystal cell		Nice specific crystal cell marker.
stage13-16		embryonic midgut crystal cell
Experiment comment: (GH17337)

in situ Images and Annotations (GH17337, dg)
Stage	Image (click thumbnail for full size image)	Body Part	Note	Comment
stage1-3		maternal
stage4-6				stripey
stage7-8		head mesoderm primordium P4 trunk mesoderm primordium P2
stage9-10		trunk mesoderm primordium head mesoderm primordium P2
stage11-12		procrystal cell
stage13-16		crystal cell
Experiment comment: exp1 weird staining combo confirmed here in weaker prep. (GH17337, dg)

in situ Images and Annotations (GH17337, cm)
Stage	Image (click thumbnail for full size image)	Body Part	Note	Comment
stage1-3		maternal		heavy staining but seems to be abundance
stage4-6		trunk mesoderm anlage in statu nascendi mesoderm anlage in statu nascendi mesectoderm anlage in statu nascendi visual anlage in statu nascendi ectoderm anlage in statu nascendi ventral ectoderm anlage in statu nascendi head mesoderm anlage in statu nascendi dorsal ectoderm anlage in statu nascendi anlage in statu nascendi subset procephalic ectoderm anlage in statu nascendi cellular blastoderm		punctate intense staining at cellular blastoderm- is it nuclear?
stage7-8		ventral nerve cord anlage procephalic ectoderm anlage mesectoderm primordium head mesoderm primordium P4 visual anlage posterior endoderm primordium P2 trunk mesoderm primordium P2 ventral ectoderm primordium P2 anterior endoderm anlage dorsal ectoderm primordium		some stripes. hard to capture becasue of intensity.
stage9-10		posterior endoderm primordium ventral ectoderm primordium anterior endoderm primordium ventral nerve cord primordium P3 mesectoderm primordium trunk mesoderm primordium crystal cell specific anlage dorsal ectoderm primordium head mesoderm primordium P2		stripes persist
stage11-12		posterior midgut primordium anterior midgut primordium procrystal cell		Nice specific crystal cell marker.
stage13-16		embryonic midgut crystal cell
Experiment comment: testing the merge of two exp for the same gene by creating a new -cm experiment (GH17337, cm)

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