Gene Information | |||||||
cDNA | Gene | Function | |||||
GH09323 LD04782 | CG18250 (Annotation Report) FBgn0034072 (Gene Report) Map: 52E2-52E3 | cytoskeletal anchor protein structural constituent of muscle | |||||
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in situ Images and Annotations (GH09323, dg) | ||||
Stage | Image (click thumbnail for full size image) | Body Part | Note | Comment |
stage1-3 | maternal | |||
stage4-6 | ubiquitous | |||
stage7-8 | ubiquitous | |||
stage9-10 | ventral ectoderm primordium procephalic ectoderm primordium faint ubiquitous anterior endoderm primordium | |||
stage11-12 | faint ubiquitous head epidermis primordium P1 brain primordium dorsal epidermis primordium ventral epidermis primordium ventral nerve cord primordium trunk mesoderm primordium | prominent in first thoracic epidermis | ||
stage13-16 | embryonic/larval muscle system embryonic central nervous system embryonic ventral epidermis ventral nerve cord embryonic/larval dorsal vessel faint ubiquitous embryonic head epidermis embryonic brain dorsal prothoracic pharyngeal muscle embryonic dorsal epidermis | i am sticking with tracheal call. | ||
Experiment comment: dotty again. subcellular confirmed but still prep too heavy (GH09323, dg) |
in situ Images and Annotations (LD04782) | ||||
Stage | Image (click thumbnail for full size image) | Body Part | Note | Comment |
stage1-3 | maternal | |||
stage4-6 | ubiquitous | d/v gradient achieved(high v)and resolves to mesectoderm. Mesectoderm not clear; if anything, gradient resolves to mesoderm, before it disappears very quickly; later reappearance in somatic mesoderm | ||
stage7-8 | ubiquitous | |||
stage9-10 | faint ubiquitous anterior endoderm primordium procephalic ectoderm primordium ventral ectoderm primordium | spot in head could be part of ant. endoderm, or part of head mesoderm. Probably no staining in mesectoderm or dor. ectoderm | ||
stage11-12 | faint ubiquitous head epidermis primordium P1 brain primordium dorsal epidermis primordium ventral epidermis primordium ventral nerve cord primordium trunk mesoderm primordium | 23782 in wrong place. Segmental clusters probably part of somatic muscle primordium | ||
stage13-16 | embryonic/larval dorsal vessel embryonic/larval muscle system dorsal prothoracic pharyngeal muscle embryonic dorsal epidermis embryonic ventral epidermis ventral nerve cord faint ubiquitous embryonic brain embryonic head epidermis | Bulk of staining most likely somatic musculature\; definit. not PNS. Trachea artefact. | ||
Experiment comment: deserving a better prep and images YES (LD04782) |