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Gene Information
cDNA	Gene	Function
GH17337	CG3400 (Annotation Report) FBgn0027621 (Gene Report) Map: 18C8-18C8	6-phosphofructo-2-kinase
Array Data (CG3400) Annotation MiniView (CG3400)

in situ Images and Annotations (GH17337)
Stage	Image (click thumbnail for full size image)	Body Part	Note	Comment
stage1-3		maternal		heavy staining but seems to be abundance
stage4-6		cellular blastoderm subset anlage in statu nascendi ectoderm anlage in statu nascendi procephalic ectoderm anlage in statu nascendi visual anlage in statu nascendi ventral ectoderm anlage in statu nascendi mesectoderm anlage in statu nascendi dorsal ectoderm anlage in statu nascendi mesoderm anlage in statu nascendi trunk mesoderm anlage in statu nascendi head mesoderm anlage in statu nascendi		punctate intense staining at cellular blastoderm- is it nuclear?
stage7-8		ventral ectoderm anlage procephalic ectoderm anlage trunk mesoderm anlage mesectoderm anlage dorsal ectoderm anlage anterior endoderm anlage posterior endoderm anlage head mesoderm anlage visual anlage ventral neuroderm anlage		some stripes. hard to capture becasue of intensity.. Seems to be almost ubiquitous expression until stage 10; then label disappears, stays longer in mesoderm and endoderm.
stage9-10		head mesoderm P2 primordium posterior endoderm primordium ventral ectoderm primordium anterior endoderm primordium mesectoderm primordium dorsal ectoderm primordium ventral nerve cord primordium crystal cell specific anlage trunk mesoderm primordium		stripes persist. Seems to be almost ubiquitous expression until stage 10; then label disappears, stays longer in mesoderm and endoderm.
stage11-12		anterior midgut primordium procrystal cell posterior midgut primordium		Nice specific crystal cell marker.
stage13-16		crystal cell embryonic midgut
Experiment comment: (GH17337)

in situ Images and Annotations (GH17337, dg) New Release
Stage	Image (click thumbnail for full size image)	Body Part	Note	Comment
stage1-3		maternal
stage4-6				stripey
stage7-8		head mesoderm anlage trunk mesoderm anlage
stage9-10		head mesoderm P2 primordium trunk mesoderm primordium
stage11-12		procrystal cell
stage13-16		crystal cell
Experiment comment: exp1 weird staining combo confirmed here in weaker prep. (GH17337, dg)

in situ Images and Annotations (GH17337, cm) New Release
Stage	Image (click thumbnail for full size image)	Body Part	Note	Comment
stage1-3		maternal		heavy staining but seems to be abundance
stage4-6		head mesoderm anlage in statu nascendi trunk mesoderm anlage in statu nascendi mesoderm anlage in statu nascendi dorsal ectoderm anlage in statu nascendi mesectoderm anlage in statu nascendi ventral ectoderm anlage in statu nascendi visual anlage in statu nascendi procephalic ectoderm anlage in statu nascendi ectoderm anlage in statu nascendi anlage in statu nascendi subset cellular blastoderm		punctate intense staining at cellular blastoderm- is it nuclear?
stage7-8		ventral neuroderm anlage visual anlage head mesoderm anlage posterior endoderm anlage anterior endoderm anlage dorsal ectoderm anlage trunk mesoderm anlage mesectoderm anlage ventral ectoderm anlage procephalic ectoderm anlage		some stripes. hard to capture becasue of intensity.
stage9-10		trunk mesoderm primordium crystal cell specific anlage ventral nerve cord primordium dorsal ectoderm primordium mesectoderm primordium anterior endoderm primordium ventral ectoderm primordium posterior endoderm primordium head mesoderm P2 primordium		stripes persist
stage11-12		posterior midgut primordium procrystal cell anterior midgut primordium		Nice specific crystal cell marker.
stage13-16		embryonic midgut crystal cell
Experiment comment: testing the merge of two exp for the same gene by creating a new -cm experiment (GH17337, cm)

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