Non-BDGP Fly EST Projects
The Exelixis ESTs were submitted by the BDGP on behalf of Exelixis. The cDNA libraries were NOT made from the y; cn bw sp strain used for genomic sequencing. Below is a description of each library.
- CK01: random primed, normalized library from mixed stage embryos, imaginal disks, and adult heads. Vector: pCDNA-sk+ standard. BDGP-assigned identifiers are "EK*".
- CK02: a subset of clones from CK01 that were sequenced from the 3' end. These were clones that did not initially overlap other contigs and where the 5' end did not read into vector (i.e. inserts >~500bp). BDGP-assigned identifiers are "EP*".
- MN08: oligodT primed from LPS induced mbn2 cell line. Vector: pBluescript. BDGP-assigned identifiers are "EN*".
- ML01 was made by Marie Lagueux in Hoffmann lab. RNA was isolated from fat body from 3rd instar larva challenged with gram+/- bacteria. CDNA was oligo-dT primed. Vector: pSport1-Tag21. BDGP-assigned identifiers are "EC*".
Unfortunately, the Exelixis cDNA clones are not available for distribution.
A testis Drosophila cDNA library was constructed by Justen Andrews in Brian Oliver's laboratory at the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) which is part of the National Institutes of Health (NIH) at Bethesda, MD, USA. Poly (A) + RNA was prepared from dissected testes from 1-5 day old y w [67cl] Drosophila melanogaster flies, after removal of the paragonia and inclusion of the ejaculatory ducts and seminal vesicles. The size fractionated cDNA (1-6kb) was directionally cloned in the Stratagene Uni-Zap XR vector using EcoRI and XhoI restriction sites. pBluescript SK (-) is produced after excision. The host strain is E.coli SOLR.
The Geneservice in the UK has approximately 3930 clones available from plates (labelled bs) numbered 3-24, 26-37, 40-42, 44-46 and 49. All clones and plates except bs49 are available on one double-spotted high density filter for screening by hybridization from them.