Selected Sequence features of Universal Proteomics Resources
Splice Donor (SD; in red) and 6xHN epitope tag from pDNR-Dual vector: CAG/GTAAGTGGTCATAATCATAATCATAATCATAATCATAATCACAAC Note: We have observed cases (<0.05%) where the splice donor is CAG/GTGAGTGGTCATAATCATAATCATAATCATAATCATAATCACAAC
Splice Acceptor (SA): CCACAG/C Note: the splice site is indicated by the forward slash (/).
Forward PCR-cloning primer (for In-Fusion PCR cloning into pDNR-Dual vector) 5'-GAAGTTATCAGTCGAC[gene-specific-sequence]-3'
Reverse PCR-cloning primer (for In-Fusion PCR cloning into pDNR-Dual vector) 5'-ATGGTCTAGAAAGCTT\[gene-specific-sequence\]-3'